Scattering Inversion Study for Suspended Label-Free Lymphocytes with Complex Fine Structures

Objective and Impact Statement . Distinguishing malignant lymphocytes from normal ones is vital in pathological examination. We proposed an inverse light scattering (ILS) method for label-free suspended with complex fine structures to identify their volumes state. Introduction Light as cell’s “fingerprint” provides valuable morphology information closely related its biophysical states. However, the detail relationships between characters are not fully understood. Methods To quantitatively of membrane nucleus main scatterers, clinical lymphocyte morphologies were modeled combining Gaussian random sphere geometry algorithm by 750 reconstructed results after confocal scanning, which allowed accurate simulation solve ILS problem. For structures, specificity study was firstly discussed (to our knowledge) considering differences only surface roughness, posture, but also ratio cytoplasm refractive index. Results The proved theoretically have good linear relationship effective area entropy forward images. Their deviations less than 3.5%. Then, experimental microsphere leukocytes showed Pearson product-moment correlation coefficients (PPMCC) this up 0.9830~0.9926. Conclusion Our inversion could be effectively applied without destructive sample pretreatments systems.